Nobilis Rismavac + CA126
Active substance
ATC code
Species
Chickens
Indications
For the active immunisation of chickens to reduce mortality, clinical signs and lesions after infection with Marek’s disease virus.
Dose to be administered and administration route
Day-old vaccination:
Inject 0.2 ml reconstituted vaccine to each bird subcutaneously in the neck or intramuscularly in the leg using a 20g x 1/2" needle with an approved repeating syringe or automatic vaccinator.
In-ovo vaccination:
Inject one dose of the reconstituted vaccine to each 18-day embryonated egg with an appropriate automatic in ovo vaccinator. The actual volume per dose may depend on the settings of the in ovo vaccination equipment. This should not be less than 50µl or more than 100µl. Depending on the volume to be administered the vaccine should be reconstituted according to the instructions below. The egg should be in an upright position with the blunt side up.
Reconstitution:
The actual volume of solvent per dose needed for reconstitution of the vaccine may depend on the number of doses per ampoule, the route of administration and for in ovo on the settings of the vaccination equipment. In the table below the volume of solvent per ampoule for the various dose-presentations, routes of administration and settings of the in ovo equipment are given.
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Volume of solvent per ampoule needed for reconstitution of the vaccine |
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Number of doses per ampoule |
sc / im (0.2 ml per dose) |
in-ovo (0.05 ml per dose) |
in-ovo (0.1 ml per dose) |
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1000 doses |
200 ml |
50 ml |
100 ml |
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2000 doses |
400 ml |
100 ml |
200 ml |
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4000 doses |
800 ml |
200 ml |
400 ml |
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5000 doses |
1000 ml |
250 ml |
500 ml |
Prior to reconstitution the vaccine is thawed. Great care should be taken - see operator warnings. Remove one ampoule from the cane and immediately replace the cane in the liquid nitrogen canister. Thaw the contents of the ampoule rapidly by immersing in water at room temperature. Do not thaw in hot or ice-cold water. Dry the ampoule and shake to disperse contents. After thawing open the ampoule immediately and draw the entire contents into a sterile 5 - 10 ml syringe using an 18 gauge needle to avoid rupturing the cells. Insert the needle through the stopper of the solvent vial (which should be at room temperature) and draw up slowly a portion of the solvent . Add the contents of the syringe to the remaining solvent . It is important that this is done slowly, allowing the vaccine to run down the side of the bottle. Gently shake the bottle as the vaccine is being mixed. Withdraw a portion of the vaccine and use to rinse the ampoule. Inject the washing back in to the solvent vial. The reconstituted vaccine must be handled gently and administered through wide gauge needles to avoid rupturing the cells. Fill the sterilised repeating syringe/automatic vaccinator according to the manufacturer’s instructions. The vial of reconstituted vaccine should be kept in an ice bath when not being used.
Adverse reactions
None
TRUSTED SOURCE
