Nobilis Gumboro D78

The vaccine is administered in the drinking water.

At least 4.0 log10 TCID50 per animal by drinking water, spray or oculo/nasal instillation. The volume used for application depends on the way of administration.

The vaccine may be delivered as a freeze-dried cake in a glass vial or as freeze-dried spheres in cups. In case of the latter presentation the cups may contain 3 up to 100 spheres depending on the required dosages and production yields. In case of the product presented in cups, do not use the product if the contents stick to the container as this indicates that the integrity of the container has been breached. Each container should be used immediately and completely after opening.

The vials should be opened under water or the content of the cup(s) should be poured into the water. In both cases mix the water containing the vaccine well before use. After reconstitution the suspension looks clear.

Reconstitution of vaccine

A. Drinking water

The vials should be opened under water or the content of the cup(s) should be poured into the water. In both cases mix the water containing the vaccine well before use. After reconstitution the suspension looks clear. The vaccine should be dissolved in cool, clean water which is free of iron and chlorine. By adding 2 gram skimmed milk powder per litre water the virus retains its activity much longer. Ensure uptake of all the vaccinemedicated water in 2 hours. Depending on the weather conditions, it may be advisable to deprive the birds of water prior to vaccination. A sufficient number of water containers to provide adequate drinking space is essential. These should be clean and free from traces of detergents and disinfectants. Dissolve 1000 doses in as many litres of water as the age of the birds in days, to a maximum of 40 litres.

The vaccine should be given in the early morning as this is the main period of drinking or the cool period on a hot day. When vaccinating larger flocks, it is advisable to start by dissolving only part of the vaccine. If vaccine is administered through a central water supply or a proportioner, great care should be taken. For numbers of birds between standard dosages, the next higher dosage should be chosen.

B. Spray method

Reconstitute the vaccine in cool, clean water, to which 2% skimmed milk may be added.

The vials should be opened under water or the content of the cup(s) should be poured into water. Chlorinated water should not be used. In both cases mix the water containing vaccine well before use. After reconstitution the suspension looks clear. The water and spray apparatus should be free from sediments, corrosion and traces of disinfectants or antiseptics. Ideally the apparatus should be used for vaccination purposes only. The volume of diluent for reconstitution should be sufficient to ensure an even distribution when sprayed onto the birds. This will vary according to the age of the birds being vaccinated and the management system, but a quantity of 1000 doses per litre water is suggested. The vaccine suspension should be sprayed evenly over the birds at a distance of 30-40 cm, preferably when the birds are sitting together in dim light. If applicable, reduce or stop ventilation to prevent loss of spray.

C. Intranasal / intra-ocular instillation

The vaccine should be dissolved in physiological saline solution (usually 30 ml per 1000 doses) and administered by means of a standardized dropper (of which the droplet size is known and consistent). The amount of diluent required for eye- or nose-drop administration depends on the number of doses and the droplet size. One drop should be applied from a height of a few centimetres onto one nostril or one eye. Ensure that the nasal drop is inhaled before freeing the bird.

Wash and disinfect hands and equipment after vaccinating. Any surplus vaccine should be destroyed by burning or by boiling.

Each container should be used immediately after opening.

Administration

Water should be withheld before vaccination. For recommendations see below under Management. Ensure that all medicated water is consumed within 1 - 2 hours. Turn on mains water when all the vaccine water has been consumed. Always make sure that there is food available when vaccinating. Birds will not drink if they have no food to eat.

Vaccination programmes

It is only necessary to apply the vaccine once to susceptible birds and this should be done as early as possible. In the field, maternal antibodies will exist in the majority of chicks, therefore, vaccination before 17 days for broiler-type birds and 21 days for replacement layers is not normally recommended where parent birds have been injected with an inactivated Gumboro vaccine.

Suggested vaccination programmes

This programme is intended only as a guide; local conditions must be allowed for.

*This may be reduced to 14 days if MDA is known to be present at only very low levels.

Management

Great care should be taken to ensure that all birds receive a full dose of vaccine when the product is administered. When used in chickens where maternal antibody still exists, the way in which this vaccine is administered is critical. The following points have been found to improve vaccine "take":

1. Water withholding should be kept to a minimum, especially in broiler birds.

Approximately half an hour is all that is required if the following management techniques are used.

2. Try to vaccinate at a time when birds are likely to be drinking, e.g. morning time for broilers, when food is in the food tracks.

3. Turn the lights down low when the water is turned off. For bell drinkers, go round the house emptying and cleaning the drinkers during the half-hour lights low period.

Mix up the vaccine according to the recommendations, and towards the end of the halfhour water withholding period, go round all the drinkers filling each with water containing vaccine. Leave the house and turn the light up. The increased light intensity will stimulate the birds to look for water and food. Therefore, it is important that food is available or the birds will not be interested in drinking. In some cases, it helps to run food tracks at the time the light intensity is increased.

4. For nipple lines a substantial volume of residual water may remain in the lines after the half-hour water withholding/dark period. It is advisable to drain the lines and prime with vaccine loaded water before allowing the birds to have access to the drinker lines. Mix up the vaccine and apply to the header tank(s). Calculate the volume of water that is left in the tank below the outlet valve and make sure you add extra vaccine to this volume of water. For example, if 10 liter remain below the outlet pipe and you are using 10 litre/1000 birds to vaccinate, add one extra vial of vaccine when mixing up vaccine for that tank. The use of this extra vaccine is important.

5. Once the vaccine has been consumed, resume management practices as normal.

This approach to vaccination will ensure a more even vaccination of the crop and will be less stressful to the birds. Performance should therefore be less adversely affected.

For further information on use of a vaccine in specific circumstances or in conjunction with other MSD vaccines consult MSD technical staff.