Huveguard NB vet.

Oral use (spray on birds, spray on feed and drinking water)

Vaccination schedule:

Spray on birds and spray on feed: administer one dose of vaccine to each chicken from 1 day of age.

Drinking water: administer one dose of vaccine to each chicken from 3 days of age.

Once the 30 ml containing 1,000 or 5,000 doses vial is opened, the entire contents must be used.

Sufficient starter feed for the chicks’ first 12-24 hours should be laid out on paper or plastic along the floor of the poultry house.

Shake the vaccine vial vigorously for 30 seconds before use to re-suspend the oocysts. Dilute the vaccine in water at the rate of approximately 1000 doses in 1 litre of water (5000 doses in 5 litres). To ensure that all oocysts are removed from the vial, rinse it out 3 times with water. Spray the oocyst suspension evenly over the surface of the feed using a coarse spray. Ensure a controlled even coverage of the total surface area of the feed available to the chickens. Agitate the applicator reservoir regularly throughout spraying to avoid settling-out of oocysts. Ensure that all available feed is treated and that the total number of doses dispensed matches the number of birds in the house.

Once the vaccine has been diluted for use it should be sprayed immediately onto feed and birds should be placed with access to the feed immediately.

When the treated allocation of feed has been consumed, routine feeding may continue.

It is recommended to monitor the feed intake and behaviour of the birds and to apply the vaccine by this method only after an adequate feed intake is expected.

For the administration of the vaccine drinkers must be used.

Provide an adequate number of drinkers and drinking space so that all chicks have access to the vaccinal water and thus can receive the correct dose. Place the drinkers evenly in the area where chicks are housed.

Water should be withheld for 2–4 hours before vaccination.

Preparation of the xanthan gum suspension:

Commercially available xanthan gum can be used.

For 1,000 doses put 3 litres of clean drinking water at room temperature in a suitable container and dissolve 5 g xanthan gum.

For 5,000 doses put 15 litres of clean drinking water at room temperature in a suitable container and dissolve 25 g xanthan gum.

Prepare the vaccine suspension as follows:

To resuspend the oocysts, shake the vaccine vial vigorously. Open the vial and pour the whole contents into clean drinking water at room temperature: 2 litres for 1,000 doses and 10 litres for 5,000 doses. To ensure that all oocysts are removed from the vial, rinse it out 3 times with water. Shake the obtained 2 litres (1,000 doses) or 10 litres (5,000 doses) of vaccine suspension and transfer gradually into the prepared xanthan gum suspension, mixing thoroughly to ensure a homogeneous suspension. Mixing the xanthan gum together with the vaccine suspension will result in a final quantity of 5 litre (for 1,000 doses) or 25 litre (for 5,000 doses) vaccine-xanthan gum suspension. Pour the vaccine-xanthan gum suspension into the drinking equipment.

Administration via spray on chickens.

For each 100 birds a dose volume of about 24 ml (0,24 ml/bird) of coarse spray suspension has to be prepared.

For spraying on chickens use Brilliant Blue (E133) coloring agent.

Preparation of the coloured diluent:

For 1000 doses put 240 ml of water in a suitable container and add Brilliant Blue (E133) colorant at a concentration of 0.01% w/v.

For 5000 doses put 1200 ml of water in a suitable container and add Brilliant Blue (E133) colorant at a concentration of 0.01% w/v.

Preparation and administration of the vaccine suspension:

Shake the 1000- or 5000- doses vial vigorously to resuspend the oocysts.

Add the entire content of the vial to the diluent and mix thoroughly. Rinse the vial 3 times with diluent to ensure that all oocysts are removed. Fill the vaccine reservoir of the spraying device with the full volume prepared. Continuously maintain homogeneity of the vaccine suspension. The pressure of the spraying device should be at 3 bars. The spraying device must provide a droplet size of ≥100 µm.

To improve the uniformity of the vaccination maintain the chicks inside the chick box for at least 1 hour in order to let them ingest all the vaccine droplets. Make sure that there is enough light so that the chickens are awake and preen themselves and each other.