Cevac MD HVT

In ovo or subcutaneous use:

In ovo:

One single injection of 0.05 ml is injected into each 18-day-old embryonated chicken egg. For in ovo application an automatic in ovo egg injector can be used. In-ovo equipment should be calibrated to ensure that a 0.05 ml dose is applied to each egg.

Subcutaneous use (preferably under the skin of the neck):

One single injection of 0.2 ml per chick is applied at one day of age.

The vaccine may be injected by automatic syringe. The 500 dose presentation is recommended for manual injection.

Overview table for recommended dilution possibilities of different presentations:

For in ovo administration:

The speed of automatic injection is at least 2500 eggs per hour. Solvent presentation of at least or more than 400 ml is recommended to prime the machine and inject for longer than 10 minutes.

The 200 ml solvent presentation may be used for manual in-ovo equipment.

For subcutaneous administration:

The usual aseptic precautions should be applied to all administration procedures. Be familiar with all safety and precautionary measures for handling liquid nitrogen in order to prevent personal injury.

Reconstitution of the vaccine:

1. After matching the dose size of the ampoules with the solvent size, quickly remove the exact number of ampoules needed from the liquid nitrogen container.

2. Draw up 2 ml of solvent into a 5 ml syringe. Use minimum 18 gauge needle.

3. Thaw rapidly the contents of the ampoules by gentle agitation in water at 2739°C.

4. As soon as they are completely thawed, open ampoules holding them at arm length in order to prevent any risk of injury should the ampoule break.

5. Once the ampoule is open, slowly draw up the content into the 5-ml sterile syringe prepared as in point 2.

6. Transfer the thawed suspension into the solvent bag. The reconstituted vaccine prepared as described is mixed by gentle agitation.

7. Withdraw a portion of the diluted vaccine from the solvent bag into the syringe and use it to rinse the ampoule. Inject it gently back into the solvent bag. Repeat once or twice.

8. The reconstituted vaccine prepared as described is mixed by gentle agitation so as to be ready for use.

Repeat the operations in point 2-7 for the appropriate number of ampoules to be thawed.

Use the reconstituted vaccine immediately, slowly mix regularly to ensure uniform suspension of cells and use within a period not exceeding 2 hours.

It should be ensured that the diluted vaccine is mixed regularly in a gentle way during the vaccination session to guarantee that the vaccine remains homogenous and that the correct virus titer is administered (e.g. when automatic in ovo injection machines are used or during long vaccination sessions).